Journal: Cell reports

Article Title: Btbd11 supports cell-type-specific synaptic function

doi: 10.1016/j.celrep.2023.112591

Figure Lengend Snippet:

Article Snippet: MK-801 maleate , Tocris , Cat# 0924.

Techniques: Binding Assay, Control, Virus, Recombinant, Titration, Plasmid Preparation, Electron Microscopy, Mass Spectrometry, Mutagenesis, Sequencing, Cloning, Variant Assay, Software, Microscopy

Journal: Pharmaceuticals

Article Title: Characterization of Non-Specific Uptake and Retention Mechanisms of [ 177 Lu]Lu-PSMA-617 in the Salivary Glands

doi: 10.3390/ph16050692

Figure Lengend Snippet: Cytotoxicity data of the ionotropic glutamate receptor antagonists kynurenic acid ( A ), memantine ( B ), capric acid ( C ); UBP302 ( D ) and MK 801 maleate ( E ). Data were normalized to the vehicle condition per compound and per cell type. Data were represented as mean cell survival percentage (%) ± standard deviation. * Indicates a p -value < 0.05; ** indicates a p -value < 0.01 and *** indicates a p -value < 0.001 for comparisons in respect to the vehicle condition per cell type.

Article Snippet: Kynurenic acid (S4719), memantine HCl (S2043), capric acid (S6906) and (-)-Dizocilpine (MK 801) maleate (S2857) were purchased from SelleckChem (Planegg, Germany).

Techniques: Standard Deviation

Journal: Pharmaceuticals

Article Title: Characterization of Non-Specific Uptake and Retention Mechanisms of [ 177 Lu]Lu-PSMA-617 in the Salivary Glands

doi: 10.3390/ph16050692

Figure Lengend Snippet: Competition study with different ionotropic glutamate receptor antagonists on PC3-PIP and A-253 cells. Cells were incubated with [ 177 Lu]Lu-PSMA-617 (5 nM) and different concentrations of kynurenic acid (0 to 1000 µM) ( A ), memantine (0 to 300 µM) ( B ), capric acid (0 to 1000 µM) ( C ), UBP302 (0 to 250 µM) ( D ) and MK 801 maleate (0 to 500 µM) ( E ). Data were normalized to the control condition per compound and cell type and presented as mean relative binding ± standard deviation. * Indicates a p -value < 0.05; ** indicates a p -value < 0.01 and *** indicates a p -value < 0.001 for comparison with the vehicle condition per cell type.

Article Snippet: Kynurenic acid (S4719), memantine HCl (S2043), capric acid (S6906) and (-)-Dizocilpine (MK 801) maleate (S2857) were purchased from SelleckChem (Planegg, Germany).

Techniques: Incubation, Control, Binding Assay, Standard Deviation, Comparison

Journal: Pharmaceuticals

Article Title: Characterization of Non-Specific Uptake and Retention Mechanisms of [ 177 Lu]Lu-PSMA-617 in the Salivary Glands

doi: 10.3390/ph16050692

Figure Lengend Snippet: Summary of the In vitro autoradiography results of the competition study. Mouse kidney ( A ) and pig salivary gland ( B – D ) issues were treated with [ 177 Lu]Lu-PSMA-617 (5 nM) and different concentrations of ionotropic glutamate receptor antagonists: 1000 µM kynurenic acid, 250 µM UBP302, 500 µM MK 801 maleate, 300 µM memantine and 1000 µM capric acid or 2-PMPA (100 µM). Percentage block (% block) is indicated underneath the autoradiography images and is represented as mean ± standard deviation.

Article Snippet: Kynurenic acid (S4719), memantine HCl (S2043), capric acid (S6906) and (-)-Dizocilpine (MK 801) maleate (S2857) were purchased from SelleckChem (Planegg, Germany).

Techniques: In Vitro, Autoradiography, Blocking Assay, Standard Deviation

Journal: Pharmaceuticals

Article Title: Characterization of Non-Specific Uptake and Retention Mechanisms of [ 177 Lu]Lu-PSMA-617 in the Salivary Glands

doi: 10.3390/ph16050692

Figure Lengend Snippet: Summary of the receptor class affinity and investigated concentrations of ionotropic and metabotropic glutamate receptor antagonists for the sulforhodamine B-based cytotoxicity assay.

Article Snippet: Kynurenic acid (S4719), memantine HCl (S2043), capric acid (S6906) and (-)-Dizocilpine (MK 801) maleate (S2857) were purchased from SelleckChem (Planegg, Germany).

Techniques:

Journal: Pharmaceuticals

Article Title: Characterization of Non-Specific Uptake and Retention Mechanisms of [ 177 Lu]Lu-PSMA-617 in the Salivary Glands

doi: 10.3390/ph16050692

Figure Lengend Snippet: Summary of the different compounds included in the cellular blocking studies and their concentrations used.

Article Snippet: Kynurenic acid (S4719), memantine HCl (S2043), capric acid (S6906) and (-)-Dizocilpine (MK 801) maleate (S2857) were purchased from SelleckChem (Planegg, Germany).

Techniques: Blocking Assay

Journal: Pharmaceuticals

Article Title: Characterization of Non-Specific Uptake and Retention Mechanisms of [ 177 Lu]Lu-PSMA-617 in the Salivary Glands

doi: 10.3390/ph16050692

Figure Lengend Snippet: Summary of the different compounds and concentrations used in the tissue blocking studies.

Article Snippet: Kynurenic acid (S4719), memantine HCl (S2043), capric acid (S6906) and (-)-Dizocilpine (MK 801) maleate (S2857) were purchased from SelleckChem (Planegg, Germany).

Techniques: Blocking Assay